Please find our helpful protocols from the link blow. If you are looking for things beyond this, feel free to contact us.

– Preparation and electroporation of oat protoplasts from cell suspension culture.  Curr Protoc Microbiol. 2007 Jun;Chapter 16:Unit 16D.3. doi: 10.1002/9780471729259.mc16d03s05.

http://www.ncbi.nlm.nih.gov/pubmed/18770615

– Protein protocols from Lab member Robyn Robderts:

Purification of GST-tagged proteins

Purification of His-tagged Proteins

Gel Extraction Protocol

Recipes:

Coomassie Blue Dye Recipe

SDS-PAGE loading dye